Calculation of Dose

Considering that the concentration of ozone dissolved in saline is approx. 20% of the given ozone concentration in gas mixture i.e. dissolution coefficient is 0,2 that means that, for example, at ozone concentration in gas mixture 1000 mcg/L in 1 L of saline the following amount of ozone will be dissolved: 0,2 x 1000 = 200 mcg O3 (calculation error not more than 10%).

During the infusion of ozonated saline without stopping ozonization, saturation of saline remains practically constant all the time of procedure (Fig. 2, area II). The dose of ozone infused to the patient is calculated as follows:

Dose (mcg) = 0,2 x concentration O3 (mcg/L) x volume of saline (L)

For example, at ozone concentration in gas mixture 1500 mcg/L and volume of saline bubbled with ozone 400 ml the dose of ozone infused the patient during the procedure = 0,2 x 1500 x 0,4 = 120 mcg O3.

During the infusion of ozonated saline after stopping ozonization it's important to consider that decomposition of ozone after stopping ozonization is described by complex exponential function, the half-time of ozone is approx. 30 minutes (Fig. 2, area II). The total dose of ozone infused to the patient within the treatment time of 30 min is 67% of the dose calculated for the procedure without stopping ozonization (calculation error not more than 10%):

Dose (mcg) = 0,67 x 0,2 x concentration O3 (mcg/L) x volume of saline (L)

For example, at ozone concentration in gas mixture 1500 mcg/L and volume of saline bubbled with ozone the dose of ozone infused to the patient during the procedure = 0,67 x 0,2 x 1500 x 0,4 = 80,4 mcg/ O3.