For today the Russian technology of ozone therapy has been completely developed differing from the Western one. Its main features are the wide use of physiological sodium chloride solution as a carrier of ozone and the use of considerably more low ozone concentrations and dosages. The tendency of Russian ozone doctors to use the possibly lowest range of therapeutically active ozone concentrations follows the most important principle of medicine - "not to harm". According to Prof. Peretyagin (President of the Russian Association of Ozone Therapy) "... ozone therapy with low ozone concentrations is a far-sighted ideology oriented to remote results and next generations of people. We all know the unhappy story with antibiotics, many pharmaceutical companies are involved in the development of next generation of antibiotics since the external and internal flora of the human body has got resistant to the previous generations. The same can happen to ozone used at the highest level because highly concentrated ozone is a powerful activator for all body systems, and if the body achieves the maximum level at once, it would adapt to this and next time would require more, but the therapeutically active and safe range of ozone concentrations is limited. So, since our low ozone concentrations work very well practically in each field of medicine, we don't see any reasons for using the higher ones. Time will tell who of us is right" was the philosophic conclusion of Prof. Peretyagin.

Ozone concentrations recommended for use by the Russian school of ozone therapy


Concentration Î3, mcg\L Concentration Range
1 … 100  Extremely low
101 … 400  Low
401 … 2000  Medium
2001 … 10000  High
> 10000

 Extremely high


The safety and efficiency of the Russian technology of ozone therapy is valid through scientific and clinical evidence in different fields of medicine.


The following mechanisms of action of medical ozone have been established and successfully proved by the Russian school of ozone therapy:

  • " owing to its high oxidating power, highly concentrated ozone used at a local level produces a bactericidal, fungicidal and viricidal effect versus the main gram-positive and gram-negative bacteria, viruses, fungi as well as some pathogenic protista;

  • " the systemic effect of medium and low ozone concentrations produced by parenteral route in medical conditions associated with hypoxia situations is based on the activation of the oxygen-dependent processes;

  • " the ozonides resulted from the ozonolysis of the unsaturated fatty acids modify the cell membranes at a structural-functional level, intensify the enzymatic systems and thereby improve the energy metabolism;

  • " the immunomodulating effect of ozone has been shown in different medical conditions;

  • " the activation of the pro- and antioxidant system of human body;

  • " along with the metabolic responses, the adaptation reactions on the part of cardio-respiratory system play a significant role in the elimination of hypoxia symptoms. It comes to an improvement of microcirculation that results in an increase in the perfusion pressure of blood, a decrease in the central and peripheral temperature, optimization of morphology and fine-structure of vascular bed, a decrease in the viscosity properties of blood, restoration of the respiratory metabolism in the lung and on the tissue level.

So, ozone administered by parenteral route involves the compensative mechanisms of human body to eliminate the disturbances of the homeostasis.


The method of intravenous ozonated saline infusions is based on the much longer contact of ozone with the internal environment of the patient that allows achieving positive therapeutic effects in different medical conditions by using considerably more low ozone concentrations and dosages compared with the Western school of ozone therapy. At the same time, the above-mentioned method allows at maximum using the powerful catalytic oxidative properties of ozone.


The correctness of the new method covering the most wide range of therapeutic action of ozone has been verified by the numerous fundamental biochemical, immunological, morphological, fine-structural, physiological and clinical studies. The use of ozonated saline solution induces an increase in the cell accumulation of biologically active compounds: phosphorylated nucleotides, regulator peptides, vitamins and amino acids.


Ozone used in low and medium concentrations induces an increase in the production of ATP, 2,3-DPG diphospoglycerate and NADPH2 in the erythrocytes that particularly results in the reduction of oxidized glutathione, antioxidant enzyme glutathione-peroxidase; of clinical importance is the resulted normalization of lipid peroxidation, stabilization of the erythrocyte membranes at a structural-functional level and an increase in the deformability of the erythrocytes.



It is important to note that the Russian technology of ozone therapy has been successfully realized thanks to the New-Generation of ozone therapy machines that allow exactly varying the dosages of ozone within the extremely low range, which was formerly considered as therapeutically inactive.


On the basis of the Russian and Western experience of ozone therapy the following important conclusions have been drawn:

  1. Ozone therapy is a non-medicamentous method of therapeutic action proved to be effective in the treatment of different kinds of diseases.

  2. Biological effects of ozone used by parenteral route are produced within the low ozone concentration range and yield positive clinical results depending on the dose of ozone used.

  3. The experience of the Russian and Western schools of ozone therapy has pointed out that the use of ozone as a medicinal agent considerably improves efficiency of drug therapy, in some cases it provides a more effective treatment than orthodox medicine and thereby reduces drug consumption. Ozone therapy helps to restore the natural oxygen-dependent reactions and processes of the sick human body.

  4. The technical features of up-to-date medical ozone generators incl. extremely precise dosage of ozone, allow using ozone within the possibly lowest range of therapeutically active concentrations like the generally accepted pharmaceutical products.

The above conclusions inspire our hope that ozone therapy has entered the new century as rapid developing science, science for praxis, science for people.